Saturday, July 05, 2008
Fabrication and characterization of a boehmite nanoparticle
W. J. Ng Æ S. Ramakrishna
Abstract
fiber membrane with sorptive characteristics intended for
removal of heavy metals was investigated. The electrospun
fiber membrane was impregnated with nano-boehmite
particles. The latter had been selected to increase surface
area of the active component. Cd (II) was chosen as the
challenge bivalent cation. The sorption capacity of the
nano-boehmite was studied as a function of pH and time.
Electrospinning was used to prepare the composite submicron
fiber membrane impregnated with boehmite
nanoparticles. The later was blended with the polymer to
produce a homogenous mixture before electrospinning.
Two polymers, the hydrophobic/PCL/and hydrophilic/
Nylon-6/, were chosen to serve as the support for the
boehmite. The nanoparticles and resulting composite
membranes were characterized using SEM, TEM, and
XRD techniques. XRD data confirmed the presence of
nano-boehmite particles in the nanofibers membrane. The
membranes so prepared were challenged with aqueous
solutions of Cd in batch isotherm tests. Atomic absorption
spectroscopy results show sorption of Cd (II) by boehmite
impregnated electospun membrane was possible and a
capacity of 0.20 mg/g was achieved.
Reference:
 | Garudadhwaj Hota Email: garud31@yahoo.com Email: garud@nitrkl.ac.in |
References
http://www.springerlink.com/content/4u5m58862368u14v/fulltext.pdf
Nanofibers and their applications in tissue engineering
Int J Nanomedicine. 2006 March; 1(1): 15–30. | PMCID: PMC2426767 |
Developing scaffolds that mimic the architecture of tissue at the nanoscale is one of the major challenges in the field of tissue engineering. The development of nanofibers has greatly enhanced the scope for fabricating scaffolds that can potentially meet this challenge. Currently, there are three techniques available for the synthesis of nanofibers: electrospinning, self-assembly, and phase separation. Of these techniques, electrospinning is the most widely studied technique and has also demonstrated the most promising results in terms of tissue engineering applications. The availability of a wide range of natural and synthetic biomaterials has broadened the scope for development of nanofibrous scaffolds, especially using the electrospinning technique. The three dimensional synthetic biodegradable scaffolds designed using nanofibers serve as an excellent framework for cell adhesion, proliferation, and differentiation. Therefore, nanofibers, irrespective of their method of synthesis, have been used as scaffolds for musculoskeletal tissue engineering (including bone, cartilage, ligament, and skeletal muscle), skin tissue engineering, vascular tissue engineering, neural tissue engineering, and as carriers for the controlled delivery of drugs, proteins, and DNA. This review summarizes the currently available techniques for nanofiber synthesis and discusses the use of nanofibers in tissue engineering and drug delivery applications.
In a more recent study, Malker et al determined the bioactivity of the PA self-assemblies by incorporating bioactive sequences within the PA. Their results indicated that the formation of the triple-helix for such a PA (ie, containing a bioactive sequence) produced an ordered structure of the bioactive sequence on the exterior of the triple helix that led to a favorable cell response (ie, cell adhesion, spreading, and proliferation) because of the similarity of the self-assembled triple helix to natural ECM. The results of this study and another previous study by Fields et al indicated that these PA structures have potential to be used as surface coatings for biomaterials to improve biocompatibility (Fields et al 1998; Malkar et al 2003).
Based on prior knowledge of PA self-assembling systems (Berndt et al 1995; Stupp et al 1997; Fields et al 1998; Yu et al 1998; Malkar et al 2003), Stupp et al designed di- and tri-block PAs that self-assembled into a rod-like architecture. By engineering the peptide head group of the PA, the authors developed a new technique for the self-assembly of PAs into nanofibers using pH control (Hartgerink et al 2001).
The synthesis of the PA involved the following salient features (Hartgerink et al 2001).
- Incorporation of phosphoserin residue to enable enhanced hydroxyapatite (HA) mineralization.
- Incorporation of RGD (Arg-Gly-Asp) peptide to increase integrin-mediated cell adhesion.
- Incorporation of four consecutive cystine residues, which form inter-molecular disulfide bonds that polymerize to provide improved structural stability.
- Incorporation of a flexible linker region consisting of three glycine residues to provide flexibility to the head group.
The preparation of nanofibers involved reduction of cystine residues of the PA to free thiol groups using dithiotheritol followed by acidification below pH 4 to cause self-assembly of the PAs into cylindrical micelles/nanofibers. The resulting nanofibers had a hydrophobic core of alkyl residues and a hydrophilic exterior lined by peptide residues. Their results indicated that the nanofibers produced by self-assembly were approximately 5–8 nm in diameter and several microns in length (see Figure 2). Hartgerink et al further investigated the mineralization potential of these nanofibers. The authors observed the formation of HA crystals that were oriented along the length of the nanofibers. This nanoscale orientation resembles the orientation of HA crystals in mineralized ECM and collagen fibers of bone tissue. Since the mineralized, self-assembled nanofibers were similar to the lowest level of the hierarchical structure of bone tissue, the authors believe that the nanofibers show potential to be used as primary building blocks for the engineering of bone or other mineralization tissue (Hartgerink et al 2001).
 | Figure 2 Transmission electron micrograph (TEM) of nanofibers formed from peptide amphiphile molecules (N terminus – C10H19O and Peptide – CCCCGG GS(PO4)RGD) that self-assembled by drying directly onto a TEM grid without adjusted pH (negatively (more ...) |
In another study, Hartgerink et al (2002) investigated the effect of variations in the molecular structure of the PAs on the self-assembled nanofibers. It was observed that modifications in the alkyl chain length of the PA alter the pH sensitivity of nanofibers, which affects self-assembly. Modification of the C- terminal region (ie, the region that is expressed on the surface of the nanofibers after self-assembly) led to changes in length and stiffness of the nanofibers. Replacement of cystine residues by alanine did not affect the self-assembly of the PAs into nanofibers. These results suggested that the self-assembled nanofibers show potential for development as novel biomaterials (Hartgerink et al 2002; Hwang et al 2002). This study also introduced three different methods of forming self-assembled PAs, including pH-controlled self-assembly, drying on surface-induced self-assembly, and divalent-ion-induced self-assembly. The study demonstrated that PAs can be self-assembled reversibly into nanofibers that result in the formation of gels through pH changes. These PA nanofibers can also be reversibly polymerized to improve their stability. The reversibility of these two procedures makes the self-assembly technique attractive as it enables the fabrication of remarkably versatile materials. In addition, this technique produces a good yield of nanofibers with low polydispersity.
Therefore, the self-assembly technique, by virtue of the modifications possible in the structure of the PA, enables a variety of self-assemblies including layered and lamellar structures, and by virtue of the aforementioned reversibilities lends flexibility to the system. Thus, the self-assembly technique shows good potential for further exploration with the goal of designing novel scaffolds for tissue engineering applications.
Reference: http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=2426767Monday, June 30, 2008
Functional Self-Assembled Nanofibers by Electrospinning
A. Greiner1 and J. H. Wendorff1
| (1) | Department of Chemistry and Center of Material Science, Philipps-University, 35032 Marburg, Germany |
Abstract Electrospinning constitutes a unique technique for the production of nanofibers
with diameters down to the range of a few nanometers. In strong contrast to conventional
fiber producing techniques, it relies on self-assembly processes driven by the Coulomb interactions
between charged elements of the fluids to be spun to nanofibers. The transition
from a macroscopic fluid object such as a droplet emerging from a die to solid nanofibers
is controlled by a set of complex physical instability processes. They give rise to extremely
high extensional deformations and strain rates during fiber formation causing among
others a high orientational order in the nanofibers as well as enhanced mechanical properties.
Electrospinning is predominantly applied to polymer based materials including
natural and synthetic polymers, but, more recently, its use has been extended towards
the production of metal, ceramic and glass nanofibers exploiting precursor routes. The
nanofibers can be functionalized during electrospinning by introducing pores, fractal
surfaces, by incorporating functional elements such as catalysts, quantum dots, drugs,
enzymes or even bacteria. The production of individual fibers, random nonwovens, or
orientationally highly ordered nonwovens is achieved by an appropriate selection of electrode
configurations. Broad areas of application exist in Material and Life Sciences for
such nanofibers, including not only optoelectronics, sensorics, catalysis, textiles, high efficiency
filters, fiber reinforcement but also tissue engineering, drug delivery, and wound
healing. The basic electrospinning process has more recently been extended towards compound
co-electrospinning and precision deposition electrospinning to further broaden
accessible fiber architectures and potential areas of application.
Keywords Co-electrospinning · Electrospinning · Fiber architectures · Functions and applications · Nanofibers · Nonwovens · Precision electrospinning
Paper Reference: http://www.springerlink.com/content/v80076257623ul64/fulltext.pdf
Fabrics made of functional nanofibers that would decompose toxic industrial chemicals into harmless byproducts.
Cornell fiber scientist Juan Hinestroza is working with the
Potential applications include safety gear for
Hinestroza, assistant professor of fiber science in the
about $875,000 will go directly to Hinestoza's work. Both grants are multi-university collaborative efforts funded through the U.S. Defense Threat Reduction Agency.
"These nanostructures could be used in creating advanced air filtration and personal protection systems against airborne chemical threats and can find many applications in buildings, airplanes as well as personal respirators," Hinestroza said.
The first project, in collaboration with
"Understanding how these charges are injected into the fibers and how they are dissipated under different environmental conditions can open an avenue to significant improvements in air filtration technology," Hinestroza said.
The position and distribution of the electrical charges on the nanofibers will be fed into computerized fluid dynamics algorithms developed by Andrey Kutznetsov of NC State to predict the trajectory of the nanoparticles challenging the filter. Hinestroza and NC State's Warren Jasper pioneered work in this area a couple of years ago.
The second project, in collaboration with the University of California-Los Angeles (UCLA), will study the incorporation of a new type of molecules -- called metal organic polyhedra and metal organic frameworks -- onto polymeric nanofibers to trap dangerous gases as toxic industrial chemicals and chemical warfare agents, then decompose them into substances that are less harmful to humans and capture them for further decontamination. The synthesis of these molecules was pioneered by Omar Yaghi of UCLA.
This project will also look into the potential toxicity of these nanofiber-nanoparticle systems to humans in collaboration with Andre Nel from
Hinestroza's research group specializes in understanding and manipulating nanoscale phenomena in fiber and polymer science. Related Information: Hinestroza Research Group
By Sheri Hall assistant communications director for the
Cornell Chronicle: Susan Lang (607) 255-3613 ssl4@cornell.edu, Media Contact: Press Relations Office (607) 255-6074 pressoffice@cornell.edu